Zebrafish kidney Histology

Zebrafish Renal Pathology: Emerging Models of Acute Kidney

  1. Further, zebrafish kidneys contain progenitors that create new nephrons after AKI. Here, we review investigations in zebrafish which have established a series of exciting renal pathology paradigms that complement existing AKI models and can be implemented to discover insights into kidney regeneration and the roles of stem cells
  2. Anatomy of the zebrafish kidney and the identification of histological stains to distinguish renal structures. (a) The adult zebrafish kidney is comprised of arborized arrangements of nephrons that share common distal late tubule segments and drain into major collecting ducts (schematic adapted from)
  3. Zebrafish as a Model of Kidney Disease Animal models have been an invaluable means to advance biomedical research as they provide experimental avenues for cellular and molecular investigations of disease pathology. The zebrafish (Danio rerio) is a good alternative to mammalian models that can be used to apply powerful genetic experimenta
  4. B and C: histology of renal tubule of NaCl-injected zebrafish at 96 hpf as a control. Cross sections of proximal pronephric tubules (PT) at 96 hpf after NaCl injection (plane of section indicated in A) reveal only few lysosomes (black droplets). The brush borders (+) of opposing tubular cells are in close contact
  5. The zebrafish has been shown to be an excellent vertebrate model for studying the roles of specific genes and signaling pathways. The sequencing of its genome and the relative ease with which gene modifications can be performed have led to the creation of numerous human disease models that can be used for testing the potential and the toxicity of new pharmaceutical compounds
  6. Sagittal section of whole zebrafish with mycobacteriosis G = granulomas in the kidney and viscera due to the bacterial infection The mere presence of a pathogen in a sick fish does not necessarily mean that it is the cause of the disease

In zebrafish, both renal function and the glomeruli structure are similar to mammals, enabling the comparison of histopathology [ 9 ]. Zebrafish is an ideal model to assess environmental toxicity [ 10 ]. In these studies, the potentially toxic compounds present in the water are rapidly absorbed through the gills Coronal section of a juvenile female zebrafish, 14 mm (6 weeks); H&E staining; H&E staining ovary location, coronal view The ovary is a paired organ, located bilaterally between the abdominal wall and the swim bladder.At the level of this section, the ovary is further bounded by the liver and intestines.Part of the pancreas is located in the anterior peritoneal cavity, between gut, liver and. Bottom: Comparison of juvenile (33 dpf) zebrafish i5 μm histology section and micro-tomographic MIPs (C and D, respectively). Juvenile scan data is of 1.43 μm 3 isotropic voxel resolution, and a MIP of 3 slices, totalling 4.29 μm thickness, is shown The zebrafish pronephric kidney provides a simple, yet powerful, model system to better understand the conserved molecular and cellular progresses that drive nephron formation, structure, and function. Bladder histology. Can be described anatomically by its 4 layers from outside inward

Zebrafish are increasingly used, as they are genetically tractable and have a basic renal anatomy comparable to mammalian kidneys with glomerular filtration and tubular filtration processing. Here, we discuss how zebrafish have advanced the study of nephrology and the mechanisms underlying kidney disease As previously mentioned, the embryonic zebrafish pronephros develops into the adult kidney known as the mesonephros.4, 5, 6 The adult zebrafish mesonephric kidney is a single, flattened structure that is adherent to the dorsal body wall via connective tissues (Fig 1, C). 86 Anatomically, the kidney consists of 3 main parts: the head, the trunk. zebrafish_kidney_anatomy 2/4 Zebrafish Kidney Anatomy Kindle File Format Zebrafish Kidney Anatomy The Zebrafish-Joseph A. Holden 2013-01-21 The zebrafish (Danio rerio) is a valuable and common model for researchers working in the fields of genetics, oncology and developmental sciences In zebrafish, polycystin 2 knockdown induces kidney cysts, hydrocephalus, left/right asymmetry defects, and strong dorsal axis curvature. Here, we show that increased notochord sheath collagen deposition in polycystin 2-deficient embryos is directly linked to axis defects

Keywords: zebrafish; anatomy; histology. HEMATOPOIETIC TISSUE In contrast to mammals, the bones of the zebrafish have no medullary cavity. Hematopoietic tissue is located in the stroma of the spleen and the interstitium of the kidney (Figures 1 and 3). In the adult zebrafish, hematopoiesis occurs primarily in th Severe kidney pathology was observed in 60 dph zebrafish, specifically glomerular dilation or degeneration, fibrosis, tubule enlargement and tubule necrosis, at a threshold of 10 ng/l EE. However, minor kidney histopathology indicated by increased pyknotic nuclei in kidney tubule and interstitial (hematopoietic) cells was detected at lower. The zebrafish kidney section is populated with epithelial lined tubules and the renal interstitium where hematopoietic elements are located. Panel A shows the staining pattern of Tg(CD41:GFP) donor fish with peroxidase-positive cells scattered among the densely packed renal interstitial cells

Atlas of Cellular Dynamics during Zebrafish Adult Kidney

Zebrafish as a Model of Kidney Disease - PubMe

Histology and Histopathology Atlas of the Zebrafish

The mesonephros becomes the adult kidney in the zebrafish and contains the nephrons responsible for filtering blood wastes and for salt and water uptake. The kidney extends the length of the body cavity (Figures 2.3, 2.4, and 10.1). The anterior or head kidney contains lymphoid, hematopoietic, steroidogenic, and endocrine cells Coronal section of a juvenile male zebrafish, 15 mm (6 weeks); H&E staining testis location, coronal view The testis is a paired organ, located bilaterally between the abdominal wall [AW] and the swim bladder.At the level of this section, the testis is further bounded by the liver and mesenchymal tissue (mainly adipose tissue); dorsally it is adjacent to the kidney and ventrally to the.

The research conducted in our laboratory has used these protocols to analyze kidney and heart development in zebrafish 3,4,5,6 N.A. et al. High-throughput zebrafish histology. Methods 39. Human diabetic patients and hyperglycaemic zebrafish illustrate no change in ELMO1 expression in the kidney. ELMO1 expression was analysed for potential changes within human kidney sections.

zebrafish cell culture, and the high divergence of mammalian and zebrafish growth factors and cytokines15,16. Development of the protocol Initially, we established a method for culturing zebrafish hema­ topoietic stem and progenitor cells (HSPCs) in suspension on top of zebrafish kidney stromal (ZKS) cells15. The ZKS cell laye Diagnosing diseases, such as cancer, requires scientists and doctors to understand how cells respond to different medical conditions. A common way of studying these microscopic cell changes is by an approach called histology: thin slices of centimeter-sized samples of tissues are taken from patients, stained to distinguish cellular components, and examined for abnormal features Abstract. Drug-induced kidney injury is a serious safety issue in drug development. In this study, we evaluated the usefulness of adult zebrafish as a small in vivo system for detecting drug-induced kidney injury. We first investigated the effects of typical nephrotoxicants, gentamicin and doxorubicin, on adult zebrafish

Acute renal failure in zebrafish: a novel system to study

Normal anatomy and histology of the adult zebrafis

The structural and functional similarity of the larval zebrafish pronephros to the human nephron, together with the recent development of easier and more precise techniques to manipulate the zebrafish genome have motivated many researchers to model human renal diseases in the zebrafish. Over the last few years, great advances have been made, not only in the modeling techniques of genetic. Histology of fish at this time point revealed tumors in multiple organs including the kidney, skin, gills, heart, intestine, and liver (Fig. 1e). Intravital imaging of adult zebrafish In mice, intravital imaging requires surgical techniques to gain access to the organs of interest [ 10 ] Zebrafish kidney marrow (ZKM), which is equivalent to the haematopoietic bone marrow of mammals, produces all major blood cell types, which morphologically resemble their mammalian counterparts. To be able to exploit the advantages of zebrafish 1. Dissection of the adult zebrafish kidney from an unfixed animal sample. Select an adult zebrafish between 3-6 months in age for dissection. Euthanize the fish by placing it into a dish with 0.2% Tricaine pH 7.0 for approximately 4-5 minutes, watching carefully to see that the gills stop moving and the heart stops beating

Bacterial kidney disease (BKD), caused by the bacterium Renibacterium salmoninarum, is a global disease problem in both wild and cultured salmonids. The disease is extremely difficult to control, information regarding the pathogenesis of R. salmoninarum is incomplete, and there is no effective treatment. Salmonids are the natural host of R. salmoninarum, but it is challenging and costly to. In addition, transgenic zebrafish with biosensors for other metabolic parameters, such as glucose, are also being validated. These transgenic zebrafish offer a versatile and accessible in vivo system to study mitochondrial dynamics and activity as well as their associated metabolism in the kidney in health and disease. Funding. NIDDK Suppor The zebrafish kidney is also a valuable system for studying AKI because, as opposed to mammals, fish can generate new nephrons throughout their life and regenerate new nephrons after injury . Diep et al., in a series of transplantation experiments, were able to identify LIM homeobox 1a-positive (lhx1a-positive) cells as adult self-renewing. Home / zebrafish histology atlas. zebrafish histology atlas. 25 enero, 2021; Sin categoría. American Society of Nephrology | Kidney Week - Abstract Details (2017) ASN's Mission. ASN leads the fight to prevent, treat, and cure kidney diseases throughout the world by educating health professionals and scientists, advancing research and innovation, communicating new knowledge, and advocating for the highest quality care for patients

In particular, the zebrafish embryo has become an important organism to study vertebrate kidney organogenesis as well as multiciliated cell (MCC) development. To visualize MCCs in the embryonic zebrafish kidney, we have developed a combined protocol of whole-mount fluorescent in situ hybridization (FISH) and whole mount immunofluorescence (IF. Zhibai Dihuang Wan (ZDW) is an eight-herbal formula of traditional Chinese medicine. Clinically, it regulated immune activity and was used to treat diabetes and renal disease. In this study, we aimed to explore the nephroprotective effect of ZDW in an aristolochic acid- (AA-) intoxicated zebrafish model. We used a green fluorescent kidney transgenic zebrafish to evaluate the nephroprotective.

Graphical representation of adult zebrafish mesonephric

General Diagnostics & Necropsy Protocols - Zebrafis

Fingerprint Dive into the research topics of 'Characterization of kidney marrow in zebrafish (Danio rerio) by using a new surgical technique.'. Together they form a unique fingerprint. Sort by Weight Alphabetically Histology. Powered by Pure,. The zebrafish kidney represents a useful model of kidney development and disease (Drummond & Davidson 2010). and the kidney of the vhl −/− larvae recapitulated clear cell histology, indicating that these fish may be used as a model of early-stage renal cell carcinoma. Histological sections revealed increased cellularity of the kidney and liver with massive infiltration of hematopoietic cells in these organs. In flow cytometry, kidney marrow cells from the transgenic fish showed a different forward scatter (FSC) and side scatter (SSC) profile compared to that of the normal zebrafish kidney marrow cells Histology of adult zebrafish (hematoxylin eosin). Furthermore, Liu et al. analyzed the profiling immune response in zebrafish intestine, skin, spleen and kidney when immersion vaccinated was used with a live attenuated V. anguillarum vaccine. Immersion, or bath vaccination, is a common practice in aquaculture, because of it being convenient. These include zebrafish, mouse, and human kidney organoids to study Alport syndrome, a genetic disorder caused by defects in the type IV collagen genes COL4A3, COL4A4, and COL4A5. To link composition to structure, we use advanced imaging approaches, including volume electron microscopy

Zebrafish kidney marrow and thymus were dissected from 3-9-mo-old transgenic fish, made into single-cell suspensions, and individual cells were FACS sorted into 96-well plates, as previously described (Moore et al., 2016a) Thus, loss of Sbds in zebrafish phenocopies much of the human disease and is associated with growth arrest and tissue atrophy, particularly of the gastrointestinal system, at the larval stage. A variety of stress responses, some associated with Tp53, contribute to pathophysiology of SDS. Introduction

Histopathology of Zebrafish (Danio rerio) in Nonclinical

Zebrafish PKD-targeted services. The zebrafish is well established as a model system for studying PKD, and Mayo Clinic has an expanding group of faculty exploring the use of zebrafish for kidney development and disease research. Five zebrafish-focused services are offered. Learn more about the core's zebrafish services. Rodent PKD-targeted. Animals acquire nutrients and energy through feeding to achieve a balance between growth and organismal health. When there is a change in nutrient acquisition, the state of growth changes and may also cause changes in the intrinsic immune system. Compensatory growth (CG), a specific growth phenomenon, involves the question of whether changes in growth can be accompanied by changes in innate. Blast-like leukemia cells predominate in the kidney marrow and efface the renal tubules (black arrow, (D). (N) Tumor incidence in CG1 tp53 del/del zebrafish (n = 134). (O) Quantitation of tumor types that form in CG1 tp53 del/del mutant zebrafish by 55 weeks of life based o Service includes: • Histologic slide preparation. • H&E and Acid-Fast stains. • Pathologist's evaluation and report. Test name and components. Zebrafish pathology. Small fish pathology: 0-6 cm (Medaka) Medium fish pathology: 7-13 cm (Bettas, Cavefish, Goldfish, Killifish) Large fish pathology: over 13 cm (Carp, large Goldfish, and others Acute kidney injury (AKI) is a rapid decline in kidney function that is associated with high mortality and morbidity 1rates, affecting 1 in 5 adults worldwide . While damaged renal tubula

The zebrafish (Danio rerio) is a freshwater fish belonging to the minnow family of the order Cypriniformes.Native to South Asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio (and thus often called a tropical fish although both tropical and subtropical).. The zebrafish is an important and widely used vertebrate model organism in scientific research, for. After myocardial infarction in the mammalian heart, millions of cardiomyocytes are lost and replaced by fibrotic scar tissue. While fibrosis is persistent in adult mammals, there are some vertebrates, including zebrafish, with the capacity for regeneration. This process does not occur in the absence of fibrosis. Here we studied subpopulations of collagen-producing cells and analyzed their fate. Zebrafish, C. elegans and Human Polycystic Kidney Disease IDENTIFYING POTENTIAL DISEASE BIOMARKERS THROUGH COMPARATIVE ANALYSIS Polycystic kidney disease is a genetic disease with no known The focus of this project is to identify cure the downstream effects of mutations in the causative genes (PKD 1 and PKD 2) of polycystic kidney disease (ADPKD)

Hematopoietic gene expression profile in zebrafish kidney marrow Huai Dong Song, Xiao Jian Sun, Min Deng, Guo Wei Zhang, Yi Zhou, Xin Yan Wu , Yan Sheng, Yi Chen, Zheng Ruan, Chun Lei Jiang, Hui Yong Fan, Leonard I. Zon, John P. Kanki, Xi Liu Ting, A. Thomas Look, Zhu Che Abstract: Kidney diseases occur at epidemic proportions worldwide. Common among kidney disease pathology is the destruction of cells that comprise the nephron, the basic structural and functional unit of the kidney organ. Interestingly, human nephrons have the capacity to replace damaged nephron cells through regeneration Zebrafish Anatomy Histology Biology Journal Impact Factor List of Journals SCI. Fish anatomy Wikipedia. Encyclopedia HealthCentral. Databases by Subject. Jobs. Optical Coherence Tomography News. What Are the Side Effects of Gallbladder Removal. The OBO Foundry. ASHG 2016 Meeting. Neuroanatomy Wikipedia. MYCN n myc Cancer Genetics Web Zebrafish embryos are completely transparent, and thus you can watch their kidneys develop in real-time. The early zebrafish kidney (called the pronephros) first appears within 24-48 hours, so experiments can be done with a rapidity unmatched in mice. Furthermore, the zebrafish pronephros is much more simple than the mammalian kidney: in fact. Atlas of Cellular Dynamics during Zebrafish Adult Kidney Regeneration KristenK.McCampbell,KristinN.Springer,andRebeccaA.Wingert traditional histology protocols for use with zebra sh renal tissues and also implemented a number of our recently developed nephron labeling methodologies [ ]. With thes

Adult zebrafish kidney showed elongated and flattened tissue that occupied the space between the vertebral column and swim bladder . The zebrafish nephron was also composed of the glomerulus, proximal and distal tubules, and collecting ducts, but no thin limb segment was observed ( Figure 1B ) Histology. For H&E staining of histological sections, adult zebrafish were fixed in 4% PFA over night. Major organs and tissues, such as brain, eyes (figures 2(A) and (C)), myotome, swim bladder, kidney, intestine and pectoral and pelvic fins (figures 2(E) and (G)), can readily be identified in the optoacoustic images, Zebrafish is a. (b) Representative liver histology image by Haemotoxylin and Eosin (H&E) staining and oil red O (ORO) staining of adult zebrafish fed with a control diet or HFD for 4 wk. The scale bar is 50 μm. (c) Representative intestinal histology image by H&E staining and ORO staining of adult zebrafish fed with a control diet or HFD for 4 wk Zebrafish Cell Stem Cell, Volume 2, Issue 2, 183-189, 7 February 2008 Embryology: Convergent extension movements and ciliary function are mediated by ofd1, a zebrafish orthologue of the human oral‐facial‐ digital type 1 syndrome gene Glomerular function and structure in ofd1 MO‐injected embryos

Histology and Histopathology Atlas of the Zebrafis

Structure & Function of Other Animals' Kidneys. Here's a very brief overview of the kidneys of animals (other than humans). All vertebrates have kidneys and the functional unit of the kidney in all species is the nephron. Freshwater fish-like the zebrafish-have a kidney which is kind of like a long tube. In zebrafish embryos (as. Samples of the kidney, liver and spleen from infected zebrafish will be tested for R. salmoninarum on KDM-2 plates, H & E and Gram stains and DFAT tissue smears. The structure of kidneys from both Renibacterium salmoninarum treated and untreated acclimated zebrafish will be analyzed and compared to wildtype zebrafish kidneys

Computational 3D histological phenotyping of whole

Deyssy Carrillo - Histology Technician. I joined the Kidney Cancer Program as Director of Research Administration in July 2020. My thesis work focused on the development of a von Willebrand factor zebrafish mutant using the CRISPR/Cas9 system. I joined the Brugarolas lab in summer of 2018, and am working on the evaluation of potential. Zebrafish whole exome sequencing RESEARCH REPORT 4447 Fig. 2. Isolation of new mutant alleles for ift172and lrrc6that cause kidney cyst formation. (A) Brightfield image of 5-dpf WT sibling and ccn1.9pd1085 homozygous mutant. Red arrowhead indicates a kidney cyst. (B) Confocal images of transverse sections of 5-dpf WT sibling and ccn1.9pd108 Although zebrafish embryos have been utilized to study ciliogenesis and to model polycystic kidney disease (PKD), adult zebrafish remain unexplored. Here, we report the generation and characterization of a zebrafish mutant of tmem67 , a homologue of the mammalian causative gene for Meckel syndrome type 3 (MKS3). Although a small population of homozygous embryos exhibited pronephric cysts, all. Instead, the zebrafish thyroid is composed of loosely scattered follicles present in soft tissue,. They are best observed in the ventral pharynx in the vicinity of the ventral aorta (Figure 9.1). The histology is similar to that of other species,. The follicles are composed of cuboidal to flat follicular cells surrounding an eosinophilic. Merino MJ, Torres-Cabala C, Pinto P, Linehan WM. The morphologic spectrum of kidney tumors in hereditary leiomyomatosis and renal cell carcinoma (HLRCC) syndrome. Am J Surg Pathol. 2007 Oct;31(10):1578-85. Kurt Schaberg MD John P Higgins MD Robert V Rouse MD Department of Pathology Stanford University School of Medicine Stanford CA 94305-534

Renal System Development - Embryolog

Figure 2 The zebrafish kidney in the embryo and adult. A, B: Live image of an embryonic zebrafish at 24 h post fertilization (hpf) and at the adult stage, respectively; C: Illustration of a zebrafish at the 24 hpf stage, with the location of the embryonic kidney, the pronephros, indicated in purple; D: Illustration of an adult zebrafish with the location of the mature kidney, the mesonephros. mesonephros serves as the embryonic kidney of mammals and the mature kidney of fish and amphibians. 1, 2. Although transient, these structures are critical for proper development of the metanephric kidney, which is the adult kidney of mammals, birds, and reptiles. 2. In mammals, the kidney arises from the primary nephric duct (Wolffian duct), a. Introduction. The zebrafish, one of the most popular animals of developmental biologists, is rapidly gaining ground as an infection and immunology model -.The ease of producing specific zebrafish mutants is an additional benefit of using this model for experimental immunology .Fish possess a well-developed, non-specific innate immune system, and phagocytes play an important role in the fish. Historically, isolation of the zebrafish adult kidney has been instrumental for studying hematopoiesis, as the kidney is the anatomical location of blood cell production in fish6,7. The kidney is composed of nephron functional units found in arborized arrangements, surrounded by hematopoietic tissue that is dispersed throughout the intervening.

Zebrafish as a model for kidney function and disease

New tides: using zebrafish to study renal regeneration

Abstract <p>A) Normal hemopoietic tissue (arrow) in interstitium of anterior kidney of zebrafish in 0 ppb group; B) depletion of hemopoietic tissue (arrow) from interstitium of anterior kidney of zebrafish in 100 ppb group at 42 d (40X).</ He became an Instructor in Pathology at the Massachusetts General Hospital/Harvard Medical School, and later, he started as an Assistant Professor in Biomedical Sciences at the New York Institute of Technology, College of Osteopathic Medicine. He studies kidney development, regeneration and pathophysiology using zebrafish as a model. Email Zebrafish as an animal model to study renal regeneration after acute kidney injury 3 rd International Conference on Translational Medicine November 03-05, 2014 Las Vegas, USA. Rebecca A Wingert. Accepted Abstracts: Transl Med. Abstract Zebrafish Research. Taking substantiated decisions in Zebrafish research. For the best result during screening, sorting, manipulation, and imaging you need to see details and structures to make the right decisions for your next steps in research. Known for outstanding optics and superb resolution, stereo microscopes and transmitted light bases.

Comparison of renal histology between zebrafish and mouseThe zebrafish kidney in the embryo and adultA Subpopulation of Label-Retaining Cells of the Kidney

Endogenous Fibroblasts Contribute to ECM Deposition in the Cryoinjured Zebrafish Heart. A population of collagen-producing cells located between the cortical and trabecular myocardium in the zebrafish heart was identified by transmission electron microscopy ().This region was further shown to harbor a cell population labeled in the -6.8kbwt1a:GFP line (hereafter termed wt1a:GFP) and to be. The zebrafish clc-k/barttin proteins are very similar to their mammalian counterparts, and both proteins are necessary to mediate chloride currents. Localization studies indicated that both proteins are exclusively expressed in the apical membranes of zebrafish kidney tubules. Knockout of clc-k resulted in embryonic lethality Component of a heteromeric calcium-permeable ion channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B. Can also form a functional, homotetrameric ion channel (By similarity). Functions as non-selective, voltage-gated cation channel (By similarity). Required for normal oscillation of Ca(2+) levels within cilia; these. Gas bubble disease (GBD) is the fish equivalent of the bends in human scuba divers. It is the result of gas coming out of solution in the bloodstream, thereby leading to the formation of emboli, especially in smaller blood vessels, including rete.Thus, lesions are common in gills, and the choroid gland of the eye Leptospirosis is an often overlooked cause of acute kidney injury that can lead to multiple organ failure and even death. The principle protein that conserved in many pathogenic leptospires is the outer membrane protein LipL32. However, the role of LipL32 in the pathogenesis of renal injury in leptospirosis is not entirely clear. Here we studied the effects of LipL32 on the developing kidney. The zebrafish kidney marrow is considered to be the organ of definitive hematopoiesis, analogous to the mammalian bone mar-row. We have sequenced 26,143 ESTs and isolated 304 cDNAs with putative full-length ORF from a zebrafish kidney marrow cDNA library. The ESTs formed 7,742 assemblies, representing bot